|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 1. Adhesion, spreading and migration responses of crest cells to LN-1 and E8 and E1' proteolytic fragments. (A) Diagram showing the structure of LN-1 and E8 and E1' proteolytic fragments. Arrowheads indicate the major integrin-binding sites situated at both ends of the 
1 chain. (B,C,G) Adhesion, spreading and migration on LN-1 and fragments at coating concentrations of 1-100 µg/ml. (D-F) Cellular morphologies after 1 hour of spreading on LN-1, E8 and E1' at 10 µg/ml. Bar, 50 µm. (H-M) Morphology and migration on (H-J) native or (K-M) heat-denatured LN-1, E8, and E1' at 10 µg/ml. Bar, 100 µm; nt, neural tube. (N) Migration tracks and morphologies of cells on LN-1 and E8 and E1' fragments. Neural tubes were explanted on the dishes and, once crest cells were seen to be separated from the neural tube (defined as t0), their migration was recorded. Migration tracks of several cells as well as their positions and shapes were plotted every hour and the total distance of migration was measured. A typical track and typical values of cell velocity (v) and of persistence of movement (p), defined as the ratio between the linear distance and the total distance covered by the cells, are indicated.
|
