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Figure 3


Fig. 3. (A-E) Morphologies over time of crest cells cultured on (A) LN-1, (B) E8, (C) E1', (D) LN-5, and (E) LN-10/11. Cells were cultured over substrates at 10 µg/ml and were photographed periodically to evaluate changes in cell shapes and densities. Numerous neurons with long neurite processes (arrowheads) are detected after 48 hours on LN-1 (LN1) only but not on E8 and E1'. On LN-10/11, neurons develop precociously after 24 hours but cannot survive up to 48 hours. Bar, 50 µm.





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