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Figure 5


Fig. 5. Spreading and migration of crest cells to LN-1 are mediated primarily by {alpha}1ß1 integrin. (A,B) Effect of function-perturbing polyclonal antibodies against {alpha}1ß1 added to the culture medium on cell spreading (A) and migration (B) on LN-1, E8, and E1' at 10 µg/ml and on LN-10/11 at 20 µg/ml. (C-E) Morphology and migration of cells cultured on E8, E1' and LN-10/11 (C, D and E, respectively) in the presence of antibodies against {alpha}1ß1 added to the culture medium at 100 µg/ml at the onset of migration. The arrow in (D) points at clusters of crest cells that failed to disperse and remained attached to the neural tube. Bar, 100 µm; nt, neural tube.





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