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Figure 3


Fig. 3. Localization of {alpha}1-tubulin and {alpha}3-tubulin as well as {alpha}4-tubulin throughout the eleventh cleavage cycle in a wild-type embryo. The constitutively expressed {alpha}1-tubulin and {alpha}3-tubulin isoforms were detected with the monoclonal DM1A primary and a Texas-Red-labeled fluorescent secondary antibody (merged images). {alpha}4-tubulin was detected by an affinity-purified rabbit polyclonal anti-{alpha}4-tubulin primary antibody and an FITC-labeled fluorescent secondary antibody (merged images). DNA appears in blue (merged images). Arrowheads indicate interpolar microtubules.





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