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Figure 6


Fig. 6. E82K-{alpha}4-tubulin is incorporated into the microtubules during in vitro tubulin-polymerization. Microtubule formation was induced in egg extracts of +/- (contains one wild-type {alpha}Tub67C gene) and Kavar18c/- females. Formed microtubules were pelleted. Egg extracts, supernatant and microtubule-containing pellet were analyzed by SDS PAGE stained with Coomassie Blue and western blot. The {alpha}1-tubulin and the {alpha}3-tubulin isoforms were detected with monoclonal DM1A primary and a Texas-Red-labeled fluorescent secondary antibody, {alpha}4-tubulin by a polyclonal anti-{alpha}4-tubulin primary and an FITC-labeled fluorescent secondary antibody.





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