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Figure 8


Fig. 8. Ectopically expressed {alpha}4-tubulin and E82K-{alpha}4-tubulin are incorporated into the microtubules in the neuroblast as shown by the encircled spindle apparatus. Ectopic expression of {alpha}4-tubulin and E82K-{alpha}4-tubulin was achieved by driving UAS-{alpha}Tub67C and UAS-Kavar18c transgenes with the nervous-system-specific elav-Gal4 driver. {alpha}1-tubulin and {alpha}3-tubulin (red) were detected with monoclonal DM1A primary and a Texas-Red-labeled fluorescent secondary antibody, {alpha}4-tubulin and E82K-{alpha}4-tubulin (both green) with a polyclonal primary anti-{alpha}4-tubulin and a FITC-labeled fluorescent secondary antibody; DNA is stained blue.





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