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Figure 2


Fig. 2. Effect of PDMP on the ER of HeLa cells expressing GFP-b(5)tail. Cells grown without doxycycline for 3 days were treated with 100 µM PDMP for 3 hours, then fixed and examined by confocal microscopy, either without further manipulation (A,D,E), or after immunostaining for calnexin (B) or giantin (C), with the use of Texas-Red-conjugated secondary antibodies. Note the presence of calnexin in the PDMP-induced structures (B), and the normal appearance of the Golgi complex (C), which is similar in GFP-expressing and non-expressing cells (the latter indicated by an arrow in the middle and lower images of C). (D) PDMP-induced ER patches at higher magnification. (E) Vertical section of a cell with PDMP-induced ER patches (lower panel) and a horizontal section of the same cell (upper panel). The red line indicates the y position along which the x-z scan was performed. Bars, 10 µm (A-C); 2 µm (D-E).





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