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Figure 7


Fig. 7. Distribution of lipid dyes between SER patches and polygonal meshwork ER. Induced HeLa TetOff were microinjected with DiIC16 (3) (two upper rows) or with FAST-DiI (two lower rows) dissolved in soybean oil. One set of cells was then exposed to PDMP whereas another set was exposed to the vector, as indicated to the left of the figure. Live cells were imaged by wide-field microscopy 3 hours later. The asterisks are placed at the centre of the microinjected oil droplets. Note in the bottom row (inset) the prevalence of the GFP fluorescence in the PDMP-induced patches compared with FAST-DiI. The boxes in the right panel of the second and fourth rows indicate areas that are enlarged (threefold) in the insets. The arrows in the bottom row indicate uncharacterised structures in which FAST-DiI is particularly concentrated. Bar, 10 µm.





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