spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. The interaction between ADAM22 and 14-3-3 isoforms is dependent on the phosphorylation of ADAM22 but not the phosphorylation of 14-3-3 proteins. (A) Lysates of HEK 293T cells expressing either full-length FLAG-tagged ADAM22 or Myc-tagged or HA-tagged 14-3-3 isoforms were treated with alkaline phosphatase (ap) as indicated. Alkaline phosphatase-treated samples were subsequently treated with sodium vanadate prior to incubation with the corresponding cell lysates. Pooled lysates were then immunoprecipitated with anti-Myc-tag or anti-HA-tag antibodies and immunoblots probed with anti-FLAG antibody to detect ADAM22 precursor protein. Immunoblots were re-probed with anti-Myc-tag or anti-HA-tag antibodies as control for 14-3-3 protein expression. (B) Lysates of HEK 293T cells expressing full length FLAG-tagged ADAM22 were immunoblotted with anti-FLAG antibody as control for protein expression levels following alkaline phosphatase treatment. (C) Lysates of HEK 293T cells expressing full length FLAG-tagged ADAM22 were immunoblotted with anti-phosphoserine (pSer) antibody and re-probed with anti-FLAG antibody.





Right arrow Return to article