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Figure 7


Fig. 7. ADAM22 variant 4 mutants lacking the ability to bind 14-3-3 proteins no longer accumulate efficiently at the membrane if RXR retention motifs are still present but co-localize with Golgi markers. Immunofluorescence of D645 glioma cells transiently transfected with (A,G) FLAG-tagged ADAM22 wild type, (B,H) FLAG-tagged ADAM22 substitution mutant lacking the ability to bind 14-3-3 proteins or (C,I) FLAG-tagged ADAM22 C-terminal deletion mutant lacking both 14-3-3 binding sites and di-arginine retention motifs. (D-F, J-L) The same cells as the panels above stained with FLAG (green), DAPI (blue), and either the Golgi markers p230 (red, D,E,F) or GM130 (red, J,K,L).





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