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Figure 4


Fig. 4. AE elongation is partially rescued in the weak afd1 alleles. (A-J) TEM analysis of synaptonemal spreads from wild-type and afd1 meiocytes. SC in the wild type at pachytene (A) with a close-up (F) showing two lateral elements (LE) and a central element (CE). The stained round patches correspond to kinetochores, the large circle is the nucleolus. Short synaptic structures in afd1-1 (B) during early prophase I with a close-up (G) showing their multilayered composition. Short synaptic structures in afd1-2 (C) with a close-up (H) showing their multilayered and entangled shape. Short synaptic structures surrounded with elongated shaggy filaments in afd1-3 (D,I). Elongated AEs in afd1-4 with synaptic structures (SS), and regions where two axial elements are coalligned similar to normal synapsis (E,J). (K-N) Non homologous synapsis in afd1-4 as shown by synapsis occurring within the same AE (red; K,L) and between different partners (red and green; M,N). (O-R) Localization of ASY1 in afd1-mutant nuclei. Patches of staining could correspond to the synaptic structures observed by TEM. Elongated AEs are clearly visible in afd1-3 and afd1-4. (S-V) Synaptic structures (S,U) and ASY1/HOP1 immunostaining (T,V) in afd1-1 and afd1-4, showing that AEs and ASY1/HOP1 are still present in late pachytene-early diplotene. Bars in A-E and K-V, 5 µm, in F-J, 1 µm.





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