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Figure 5


Fig. 5. Bouquet formation is rescued in afd1-4, but homologous pairing and RAD51 distribution are impaired in all the afd1 alleles. (A-D) Homologous pairing in wild-type and afd1 nuclei during pachytene using FISH with 5S rRNA probe (green) and DAPI staining (red). (A) Paired 5S rRNA focus in the wild type. (B-D) Unpaired and doubled 5S rRNA foci in afd1-1, afd1-3, afd1-4. (E-H) Bouquet formation in wild-type and afd1 mutant nuclei at zygotene shown by FISH with telomere probe (green). (E) Bouquet in wild type. (F,G) Dispersed telomeres in afd1-1 and afd1-3 nuclei. (H) Bouquet in afd1-4 nuclei. (I-L) RAD51 foci (green) morphology and distribution during pachytene monitored by immunofluorescence. (I) Multiple round RAD51 foci in wild-type nuclei during pachytene; some foci are paired (arrowheads). (J-L) Cluster of RAD51 foci in afd1-1, afd1-3 and afd1-4. Bar, 5 µm.





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