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Figure 2


Fig. 2. Palladin knockdown decreases PDGF-induced ruffle formation. (A) A7r5 cells transfected with control pSuper RNAi and pSuper RNAi targeting palladin were plated on fibronectin overnight and serum-starved for 2 hours prior to PDGF treatment. Cells were fixed, permeabilized and stained with TRITC-phalloidin. Transfected cells (green fluorescence) were detected by the presence of GFP encoded in the pSuper vector. (B) The proportion of cells developing ruffles after PDGF stimulation is shown for cells transfected with palladin siRNA (knockdown, KD, 10±2%) and transfected with control siRNA (C, 50±5%). Results are representative of three independent experiments in which at least 100 transfected cells were counted. Bar, 10 µm.





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