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Fig. 3. Palladin localizes to PDBu-induced podosomes. A7r5 cells were plated on fibronectin and treated with the phorbol ester PDBu. After fixation, endogenous palladin was detected by immunofluorescence. Co-labeling with TRITC-phalloidin and polyclonal anti-palladin antibody reveals that palladin co-localizes with actin. Top: low magnification image. Bottom: high magnification image to show detail.