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Figure 4


Fig. 4. Palladin knockdown decreases PDBu-induced podosome formation. (A) A7r5 cells transfected with control pSuper RNAi and pSuper RNAi targeting palladin were plated on fibronectin overnight and treated with PDBu. Cells were fixed, permeabilized and stained with TRITC-phalloidin. Transfected cells (green fluorescence) were detected by the presence of GFP encoded in pSuper. Top: low magnification image. Bottom: high magnification image to show detail. (B) The proportion of cells developing podosomes after PDBu stimulation is shown for cells transfected with palladin siRNA (knockdown, KD, 20±6%) and transfected with control siRNA (C, 42±8%). Results are representative of three independent experiments in which at least 100 transfected cells were counted.





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