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Fig. 6. Yeast two-hybrid analysis of palladin-Eps8 interaction. (A) Mapping of the palladin-binding site in Eps8. Yeast cells were co-transformed with various Eps8 fragments in prey vector and full-length palladin in bait vector. (B) Mapping of the Eps8 binding site in palladin. Palladin C-terminal, N-terminal and overlapping N-terminal constructs were tested for interaction with Eps8. Growth was estimated after 5 to 7 days of incubation at 30°C. The formation of colonies secreting X-alpha-gal was taken as an indication of an interaction between the expressed proteins and is noted by `+'. No interaction between expressed proteins is noted with `-'. Boxes shown are the regions mapped to be essential for the interaction in each analysis. Controls were systematically performed for each construct with the opposite vectors without insert. Each experiment was repeated at least twice.
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