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Figure 8


Fig. 8. Rac activity is decreased in palladin knockdown cells. (A) Western blot analysis of whole cell lysates of HeLa cells transfected with control siRNA (C) or siRNA targeting palladin (knockdown, KD). Active Rac was specifically pulled down from cell lysates containing equal amounts of proteins with immobilized recombinant PAK-CRIB and analyzed by western blotting using anti-Rac antibody. A representative experiment is shown. (B) The level of active Rac was quantified by densitometric analysis of western blots and the amount of active Rac was normalized to the amount of total Rac. Rac activation was quantified from three independent assays.





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