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Figure 1


Fig. 1. Human separase is phosphorylated on S1126. (A) Schematic representation of human separase showing the ARM-repeat domain, the active and inactive caspase-like domains, and the regulatory region, which contains S1126 and the cyclin B1 binding domain. The catalytic cysteine C2029 and the auto-cleavage sites (AC) are shown. (B) Dot blot showing that the separase antibody recognises the phosphorylated and non-phosphorylated peptides, whereas the P-S1126 antibody only detects the phosphorylated peptide. (C) Immunoprecipitations of Myc-tagged proteins showing that the separase antibody recognises both WT and separase S:A, whereas the P-S1126 antibody only detects WT. The band marked by the arrow head represents full-length separase, the asterisk marks the N-terminal auto-cleavage product.





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