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Figure 4


Fig. 4. The separase S1126A mutation induces mitotic arrest. (A) Immunoblot showing tet-induced expression of Myc-tagged WT and separase S:A. Notice the increase in securin following separase induction. (B) Histograms showing accumulation of cells with DNA content >=4N cells 24 hour post-induction of separase S:A. Numbers represent mitotic index as determined by MPM-2 staining. (C) Immunoblot of tet+ cells showing equivalent expression of WT, S1126A, C2029A and the S1126A-C2029A double mutant. (D,E) Bar graphs showing the time spent in mitosis based on phase-contrast time-lapse analysis.





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