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Figure 5


Fig. 5. Cyclin B1 binding is required to prevent premature activation of separase. (A) Immunoprecipitations of Myc-tagged proteins from cell lines harbouring four separase transgenes blotted as indicated. (B) DNA content profiles showing G2/M defect 24 hours post-induction of separase mutants. (C) Box-plots of phase-contrast time-lapse microscopy analysis showing mitotic arrest phenotype following induction of separase mutants that do not bind cyclin B1. WT and separase S:A are shown for comparison. (D) Bar graph quantitating the number of metaphase spreads with separated chromatids 8 hours post-induction.





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