spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 7


Fig. 7. Separase S1126A uncouples chromatid disjunction from mitotic exit. (A) GFP histone time-lapse analysis showing a normal mitosis in a WT separase cell. (B) Prolonged mitosis in a separase S:A cell, showing rapid chromatid disjunction followed by realignment of separated chromatids. (C) Separase S:A cell showing chromatid disjunction with unaligned chromosomes (arrow heads). (D) Quantitation of normal and abnormal mitoses in WT and separase S:A populations. (E) Bar graphs showing the time from nuclear envelope breakdown to metaphase and from metaphase to sister chromatid separation. Cells expressing WT separase are sub-divided into those that performed a normal mitosis (green), or an abnormal mitosis (blue), whereas all separase S:A cells are plotted together (red). Notice that metaphase in separase S:A cells is defined as the point prior to chromatid disjunction when most or all of the chromosomes have aligned.





Right arrow Return to article