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Figure 8


Fig. 8. Separase overexpression sequesters securin in the cytoplasm. Immunofluorescence images showing that tet-induced expression of WT separase (A) and separase S:A (B) results in the endogenous securin accumulating in the cytoplasm of interphase cells. Notice that, whereas securin is not present in anaphase WT cells (arrow head), it is detectable in separase S:A cells that have undergone a premature loss of cohesion (arrows).





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