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Figure 4


Fig. 4. BNIP-H is expressed in differentiating P19 cells. (A) Lysates of untreated and retinoic acid (RA)-treated P19 cells were prepared and analysed by western blots with BNIP-H antibody (i) or glutaminase antiserum (ii). Arrow in ii indicates KGA as one of several isoforms detected in these cells by the polyclonal antiserum. The blots were then stripped and reprobed with ß-tubulin antibody to normalise for their loading. Lanes in i were derived from the same blot after removal of unrelated lanes in between. (B,C) Retinoic acid-treated P19 cells were fixed, permeabilised and probed with BNIP-H antibody, glutaminase antiserum or neurofilament-160 antibody, followed by appropriate fluorophore-conjugated secondary antibodies and analysed by confocal microscopy as described in Materials and Methods. Two sets of representative images were taken (i and ii) and each was merged (right panels) to compare their relative distribution and localisation. Bars, 10 µm.





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