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Figure 6

Figure 6


Fig. 6. BNIP-H relocalises KGA to neurite terminals independently of mitochondria. PC12 cells were untransfected (A) or transfected with the indicated epitope-tagged expression plasmids (B-E) and stimulated with NGF for 24 hours. (A) Cells were visualised under bright field to indicate the neurite-outgrowth induced after NGF treatment. (B-E) PC12 cells overexpressing the indicated proteins were fixed, permeabilised and detected by appropriate anti-FLAG or anti-HA, followed by appropriate fluorophore-conjugated secondary antibodies and confocal microscopy detection as described in the Materials and Methods. Mitochondria were stained with MitoTracker dye. Representative images are shown either in their individual colour, merged or in bright fields. Arrows in E indicate the neurite terminals to which KGA had been relocalised by BNIP-H. However, there is no increase in the concentration of mitochondria in these sites. Bars, 20 µm (A-D); 40 µm (E).





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