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Fig. 11. Effect of pifithrin-
on HIF-1 and p53 protein level and transcriptional activity in HepG2 cells. (A,B) Cells were incubated for 5 hours under normoxia or hypoxia in the presence of different concentrations (µM) of pifithrin- or 50 µM DRB. Total cell lysates were tested for HIF-1 (A), total p53 (B) and -tubulin by western blotting. (C,D) Cells were incubated for 6 hours under normoxia or hypoxia in the presence of 25 µM pifithrin and/or 50 µM DRB. Real-time PCR analysis of the aldolase (C) and p21 (D) mRNA level was performed. Relative aldolase or p21 mRNA levels were calculated as the ratio of aldolase or p21 expression in hypoxia-incubated cells to these in control normoxic cells, both normalized to -tubulin levels.
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