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Figure 8


Fig. 8. Effect of nutlin and TBB on MDM2, HIF-1{alpha} and p53 protein levels in HepG2 cells. Cells were incubated for 5 hours under normoxia or hypoxia in the presence or the absence of 10 µM TBB or of 5 µM nutlin. Total cell lysates were tested for HIF-1{alpha}, p53, MDM2 and {alpha}-tubulin protein expression by western blotting.





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