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Figure 2


Fig. 2. Mesenchyme-free epithelial cells move relative to each other. (A) Schematic diagram indicating the region of isolated E12 SMG epithelial cells analyzed by time-lapse microscopy in supplementary material Movie 3. (B) First frame of time-lapse Movie 3 with the basement membrane marked by a white dashed line. The yellow dashed box indicates the region examined at higher magnification in C and D. (C) Three frames from time-lapse Movie 3 with cells analyzed in D marked with arrowheads. (D) Frames from C were converted to a binary image (dark green overlay), and cell centroids were calculated automatically (yellow points). The distance between the two cell centroids (cyan dashed line) increased over time, indicating that the cells were migrating: 25.6 µm (0:00), 29.1 µm (0:24), and 38.6 µm (0:48). The velocity varied between frames: 8.7 µm/hour and 23.7 µm/hour. Bars, 20 µm. Time, hours:minutes.





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