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Figure 3


Fig. 3. AF6i3 knockdown in MCF10A cells accelerates epithelial wound closure. (A) (left) Western blot analysis of whole-cell lysate from MCF10A cells stably expressing AF6 shRNA (AF6i3 knockdown) or control shRNA (control). (Right) Immunofluorescence images of cell monolayers. Bar, 20 µm. (B) Light-microscopy images of control and AF6i3-knockdown cells immediately after wounding (0 h; top) and 13 hours later (13 h; bottom). Bar, 0.5 mm. (C) Graphic representation of the covered wound area 13 hours after wounding. Values for ±s.d. were derived from three independent experiments. Significant differences (p) were determined with the paired two-tailed Student's t-test.





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