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Figure 8


Fig. 8. AF6i3 knockdown results in reduced association of E-cadherin with the actin cytoskeleton and with p120-catenin during wound closure. (A) Western blot analysis showing the Triton-insoluble fraction of AF6i3-knockdown and control cells prior to migration (0 h) and 6 hours after wounding. (B) Graphic representation of western blot analysis shown in A. Values were normalized to the values for control cells. Values for ±s.d. were derived from three independent experiments. (C) Coimmunoprecipitation of E-cadherin and ß-catenin with p120-catenin in AF6i3-knockdown and control cells 6 hours after wounding. Anti-Flag antibody was used as a negative control. (D) Graphic representation of coimmunoprecipitation shown in C. Values were normalized to the values for control cells. Values for ±s.d. were derived from three independent experiments. (E) Wound healing assay of AF6i3-knockdown and control cells in the presence of indicated inhibitors. Graphic representation of covered wound area 13 hours after wounding is depicted. Values for ±s.d. were derived from three independent experiments. (F) Western blot analysis of AF6i3-knockdown and control cell lysates during wound healing assay. One representative experiment out of three is shown.





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