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Fig. 1. Structure of the cbs gene and its protein product. The cbs gene is located in the 50A region on the right arm of the second chromosome in Drosophila. (A) Transcript map of cbs, showing the location of cnn (green), the two promoters (P1 and P2) and the exon structure (red) of five transcripts that potentially produce three different protein isoforms. UTRs are in pink, except the unique UTR in the short transcript, which is in yellow. Both promoters produce transcripts that contain a GRIP domain spanning exons 5 and 6. (B) Alignment of the GRIP domains from Cbs and Golgin-97 showing identical residues in dark grey and similar residues in boxes. The ten amino acids that are eliminated from the non-GRIP, long isoform produced by two transcripts are underlined. (C) The predicted secondary structure of Cbs is 60% coiled-coil (red), with short disordered regions (blue) interrupting the coils. No coiled-coil predictions were provided for the 
-helical parts at either end (black). The GRIP domain (yellow) is near the C-terminus of Cbs, followed by 18 amino acids. Putative post-translational modifications of Cbs include nine threonine phosphorylation sites (P) and three myristylation sites (purple line). (D) Western blot showing the bands identified by guinea pig anti-Cbs whole serum and rabbit anti--tubulin 84B as a control. The upper band in the Cbs lane probably represents both of the larger isoforms, which would be expected to migrate together. The lower, fainter band runs in a position predicted for the truncated shorter isoform.
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