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Fig. 3. The fluorescence intensity of Cbs cycles during syncytial mitotic cycles. In wild-type embryos (green bars) Cbs FI is the highest during prophase and drops by approximately 50% during metaphase and early anaphase, before increasing during late anaphase and telophase. In Cbs RNAi depletion experiments (red bars) embryos with severe defects all have FI levels that are significantly lower at all stages of mitosis. The variation among nuclei in both wild-type and Cbs RNAi embryos fluctuates in a similar pattern. In Arf72A1-mutant embryos (blue bars) FI values are relatively constant throughout mitosis, as is the variation among nuclei.