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Fig. 5. Cbs colocalizes with Lva during chromosome congression and cellularization. The localization of Cbs and Lva in wild-type (OreR) syncytial embryos was determined with antibodies directed against Lva (green), Cbs (red) and DNA was stained with TOTO-3. (A-D) Lva colocalizes with a subset of Cbs (yellow in B,C) on DNA during chromosome congression and is sensitive to microtubule stability. Both, our initial fixation method (A,B) and the formaldehyde preparations that are pretreated with taxol (C,D) preserve this colocalization, although the FI is strongest with our initial method. (E,F) In a top view of an embryo during early cellularization Cbs becomes concentrated in the pericentriolar region (E) where Cbs colocalizes with Lva (yellow in F). (G,H) In a lateral view of the slow phase of cellularization, nuclei become columnar in shape and Cbs extends down over the lateral surface of nuclei (H), as Lva moves in a basal to apical direction (Sisson et al., 2000). Cbs and Lva colocalize weakly along the lateral sides of nuclei, and strongly at the apical surface of nuclei (yellow in G). We do not find large Lva vesicles in the lateral domain of Cbs, and Cbs is not present above nuclei at the region where Lva is incorporated into membranes of growing furrows. Bar, 20 µM.
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