QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 2. High-affinity specific binding of GFa and CPp to HEK-293 cells. (A) ¹²⁵I-GFa (150,000 cpm/sample) was incubated with HEK-293 cells with increasing concentrations of unlabeled effectors for 3 hours at 4°C. After extensive washing the cell-surface-associated radioactivity was determined. The ¹²⁵I-GFa-specific binding to cells in the absence of competitor was 1300 cpm. Results are presented as the mean of specific binding ± s.d. in percent. Experiments were carried out in duplicate and data shown here are representative of three independent experiments. (B) ¹²⁵I-CPp (150,000 cpm/sample) was incubated with HEK-293 or HEK-293/uPAR-12 cells with increasing concentrations of the indicated unlabeled effectors for 3 hours at 4°C as described above. The ¹²⁵I-CPp-specific binding to cells in the absence of competitor was 2270 cpm. Results are presented as the mean of specific binding ± s.d. in percent. Experiments were carried out in duplicate and data shown are representative of three independent experiments. (C) 50 nM uPA 1-158 was incubated with 5 µl of conditioned medium from LB6 cells expressing soluble uPAR (suPAR) for 1 hour at 37°C with or without the indicated concentrations of CPp or GFDp. Samples were crosslinked with 1 mM DSS for 15 minutes on ice and loaded onto a 12.5% SDS-PAGE. Arrows indicate the suPAR and the complex between suPAR and uPA 1-158 (suPAR/uPA 1-158).

Return to article