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Figure 2


Fig. 2. Differential phosphorylation of Cx43 localized at intercalated disks vs lateral edges of myocytes in the normal and ischemic heart. The localization of total Cx43 (Sigma C6219) and pS325/328/330-Cx43 in normal and ischemic heart is shown. Notice the increase in Cx43 at the lateral edges of the myocytes of the ischemic heart. Lateral edge Cx43 was not detectably phosphorylated at residues S325, S328 and/or S330. By contrast, Cx43 at the intercalated disks of ischemic heart retained phosphorylation at residues S325, S328 and/or S330 (indicated by arrowheads). Bar, 20 µm.





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