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Figure 3


Fig. 3. The pS325/328/330-Cx43 antibody is specific for the P2 isoform of Cx43 and pS328/330. (A) Equal amounts of protein lysate from fibroblasts expressing wild-type Cx43 (WT) and Cx43-TM (TM, clone A) were either treated with alkaline phosphatase (+AP) or untreated and probed in a western blot with Cx43NT1 (left panel, Total Cx43) and the pS325/328/330-Cx43 (right panel) antibodies on the same blot. Molecular mass standards in kDa are marked on the left side and the P2 isoform is marked with an asterisk. (B) The amount of antibody bound to the different peptides representing singly, doubly and triply phosphorylated Cx43 linked to an ELISA well (open bars) is shown together with the ability of these peptides to compete with Cx43 present in heart lysates in a western immunoblot format (filled bars). Error bars represent the mean ± s.d.





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