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Figure 4


Fig. 4. Cx43-TM-expressing cells do not phosphorylate Cx43 to yield the P2 isoform and contain less Triton-insoluble (TI) Cx43. Whole-cell (WC) and TI cellular lysates from cells expressing wild-type Cx43 (WT) or three Cx43-TM clones (TM-A, TM-B, and TM-C) were western blotted and probed for total Cx43 with the Cx43NT1 antibody. A darker exposure of lysates of the TM-C clone is shown. Migration positions of a 50- and 36-kDa marker are indicated with an asterisk and the vinculin loading controls are indicated with a V. The Triton-insoluble vinculin could be associated with the cytoskeleton or adhesive junctions.





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