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Fig. 6. Morphology and motility of microprocesses and growth cones are not acutely affected by aggrecan. Tectal cells were cultivated on immobilized contactin 1 and pretreated for 20 hours with TN-R (10 µg/ml). (A) Phase-contrast images were taken before (upper panel), 15 minutes after (middle panel) and 1 hour after (lower panel) the addition of aggrecan (20 µg/ml) under time-lapse control. Bar, 10 µm. For evaluation of motility within a 1-minute intervall, compare left with right image. (B) For quantification, growth cones were scored according to their size (see Materials and Methods) and the percentage of cells with an enlarged growth cone was calculated in the absence or presence of aggrecan (20 µg/ml) for 15 minutes or 1 hour as indicated. The protein in solution and the incubation periods are given at the bottom. Error bars indicate s.e.m. In contrast to Figs 2 and 3, growth cone size was scored regardless of the morphology of the neurite. Addition of aggrecan does neither affect the motility of microprocesses and growth cones of tectal cells after pretreatment with TN-R (see arrows in A) nor significantly reduce the high percentage of large and complex growth cones.
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