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Figure 1


Fig. 1. Microtubule (MT) and plus-end-tracking protein (+TIP) distribution in the budding yeast mating pathway. (A) MT morphologies through the first zygotic division. Upper panel shows fluorescent images of GFP fused to tubulin (GFP-Tub1p) in the mating pathway. Lower panel are corresponding DIC images. (B) +TIP localization in pheromone-treated cells. Schematic of mating cell is shown on the left. Formation of the shmoo after cell polarization allows MT plus ends to interact with the shmoo tip. Bik1p-3xGFP localizes to MT plus ends in the shmoo tip, free MT plus ends, and to the SPB (right, bright-field image is displayed in lower right corner). Bars, 2 µm.





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