QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 5. No effect of PD98059 was seen on pH_i and P-MAPK. (A) Change in pH_i. Eggs were fertilized ( ${blacktriangleup}$ , ${triangleup}$ ) or not ( $bullet$ , ${circ}$ ) in the presence ( ${triangleup}$ , $bullet$ ) or not ( ${blacktriangleup}$ , $bullet$ ) of 50 µM PD98059. PD98059 did not modify the increase in pH_i observed after fertilization, nor did it change the small acidification observed in unfertilized eggs. (B) Changes in the phosphorylation of the ERK-LP detected by western blot using the anti-phosphoMAPK42/44 antibody after fertilization (F) or treatment with 10 mM ammonium (A) or with 10 µM A23187 (I). MAPK was rapidly dephosphorylated after fertilization or activation with the Ca²⁺ ionophore, but no change was observed after treatment with ammonium.

Return to article