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Fig. 1. TIRF microscopy images of a CV1 cell co-transfected with GFP-GAK and mRFP-clathrin. (A) A CV1 cell was simultaneously imaged for GFP-GAK (left) and mRFP-clathrin (middle) and images were merged (right). (B) Magnified time-lapse sequence of boxed areas from GFP-GAK and mRFP-clathrin images of A showing the behavior of GAK and clathrin. GAK and its corresponding clathrin puncta are indicated by the arrow, arrowhead and pointer.