spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Recruitment of GAK or auxilin to CCPs. Fluorescence intensity of GFP-GAK and mRFP-clathrin observed under a TIRF microscope was analyzed by Metamorph as described in the Materials and Methods. (A) Time course of the change in fluorescence intensities of GFP-GAK (bullet) and GFP-auxilin ({circ}). (B) Change in fluorescence intensities of GFP-GAK ({circ}) and mRFP-clathrin (bullet) as a function of time; measured from 56 puncta in 9 cells. (C) Change in fluorescence intensities of GFP-auxilin ({circ}) relative to mRFP-clathrin (bullet) as a function of time; measured from 41 puncta in 7 cells. Sequential images were taken one frame per second for 2 minutes. The zero time point was set to the time at which the GAK or auxilin reached its maximum.





Right arrow Return to article