spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. GFP-GAK and mRFP-clathrin recruitment and disappearance from the plasma membrane imaged using evanescent and epifluorescence microscopy. CV1 cells, co-transfected with GAK and clathrin, were simultaneously imaged at a rate of 1 frame per second for a total of 2 minutes. (A) Epifluorescence images from time-lapse sequence of GFP-GAK and mRFP-clathrin. GAK and its corresponding clathrin puncta are shown by the arrow and arrowhead. (B) Time course of the change in fluorescence intensities of GFP-GAK ({circ}) and mRFP-clathrin (bullet) imaged in the evanescent field from 11 events in 5 cells. (C) Time course of the change in fluorescence intensities of GFP-GAK ({circ}) and mRFP-clathrin (bullet) imaged in the epifluorescent field using the same events that were identified in the evanescent field. The data were normalized so that the peak of GAK fluorescence was set to zero.





Right arrow Return to article