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Figure 9


Fig. 9. Kymographs of GAK and mRFP-clathrin obtained under different experimental conditions. GFP-GAK and mRFP-clathrin were imaged simultaneously by TIRF microscopy. Kymographs were generated using Metamorph software by taking a rectangular region and assembling this region in a side-by-side chronological order to form a montage. TIRF images were acquired under following conditions: in medium (DMEM and FBS; Control) in K+-depletion medium (K+-depletion), in hypertonic sucrose solution (sucrose) and in medium containing 2.5 µM LatA.





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