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Fig. 6. Section in situ hybridization (A,B) and semi-quantitative RT-PCR (C) analyses showing the expression profiles of Sox17, Sox18 and Sox7 in the livers, kidneys and other organs of the wildtype mice at P7 and adult stages. (A) The sections of the wildtype livers at P7, showing that Sox17 and Sox18, but not Sox7, are expressed in the endothelial cells of central veins (cv) and their closely associated sinusoidal vessels (arrowheads). Lower panels show the magnified view of central veins. cv, central veins; hc, hematopoietic cell. (B) The sagittal sections of the wildtype kidneys at P7, showing the expressions of Sox17 and Sox18, but not Sox7, in some populations of the outer medulla vasa recta (arrowheads and inset in bottom panles). Bottom panels show the magnified view of the outer medulla region and the outer medulla vasa recta (inset). ct, cortex; gl; glomerulus; im, inner medulla; om, outer medulla. (C) Semi-quantitative RT-PCR analyses showing relative expression levels of Sox17, Sox18 and Sox7 in various tissues of the wild-type mice at P7 and adult stages. Arrows indicate position of Sox products. Bars indicate position of G3pdh products as an internal control. Bars, 50 µm.

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