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Figure 9


Fig. 9. Quantitative analyses showing a drastic reduction of capillary densities of the affected Sox17+/--Sox18-/- livers and kidneys at P7. (A,B) Anti-PECAM-1 staining visualizes sparse sinusoids in the livers (A) and poorly-developed vascular bundles in kidney (arrowheads in B) of the severe Sox17+/--Sox18-/- group at P7. (C,D) Quantitative analyses showing the densities (C) and the numbers (D) of PECAM-positive microvasculatures per unit area in the surface (S) and proximal (P) zones of the livers, and in the cortex (C), outer medulla (OM) and inner medulla (IM) of the kidneys in the severe phenotype Sox17+/--Sox18-/- (solid bar) and Sox18-/- (open bar) groups at P7. Significance levels are indicated by **P<0.01 and *P<0.05. asterisk, non-specific staining in the apical surface of the hepatic capsule; ct, cortex; cv, central vein; gl, glomerulus; ia, interlobular artery; im, inner medulla; om, outer medulla. Bar, 50 µm.





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