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Figure 4


Fig. 4. Localization of full-length SR45 and deletion mutants of SR45 fused to GFP. (A) Schematic diagram of the GFP-tagged SR45 deletion mutants. RS1 and RS2, arginine-serine-rich domain 1 and domain 2, respectively; RRM, RNA-recognition motif. Asterisks on full-length GFP model indicate location of predicted nuclear localization signals (NLS). Table on the right side summarizes the subcellular and subnuclear localization of GFP-tagged full length and deletion mutants of SR45. N, Nuclear; Cyt, Cytoplasmic; Nucleo, Nucleoplasmic; Spec, Speckle. (B) Arabidopsis mesophyll protoplasts were transiently transfected with vectors expressing the indicated GFP-tagged SR45 mutant and examined by confocal microscopy. Shown are single confocal optical sections. Fourth column of panels displays the expression of GFP-fusions in several protoplasts in a single-view field at low magnification (40x); bar, 100 µm. First, second and third column of panels show fluorescence images, chlorophyll autofluorescence images and their respective merged images (as indicated above each panel); bars, 10 µm. As expected, full-length SR45 localized in a speckled pattern in the nucleus. The rest of the mutants displayed a range of distributions, summarized in A.





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