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Figure 1


Fig. 1. Schematic representation of human Pex19p constructs. (A-C) Representative Pex19p constructs of deletions from C-terminus, N-terminus, and both terminal regions, respectively, used in this study. Numbers designate the positions in the amino acid sequence. Two tandem epitopes of hemagglutinin (HA2) were tagged to the N-terminus of respective Pex19p variants. Several mutants with shorter sequences were fused to hexa-Myc tag, Myc6, and HA2. Farnesylation CAAx motif is shaded. The activities of the constructs in restoring peroxisomes in pex19 ZP119 cells and translocating to peroxisomal membranes in CHO-K1 are summarized on the right: +++, strong; ++, medial; +, positive; +/-; weakly positive; -, negative.





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