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Figure 4


Fig. 4. Interacting region of Pex19p with Pex3p and Pex26p. Pex19p interaction was assessed by yeast two-hybrid assay. Host strain MaV203 was transformed with two plasmids, one encoding the DNA-binding domain (BD) of Gal4p fused to the full-length rat Pex3p (A) or human Pex26p (B) and the other encoding the Gal4p transactivation domain (AD) fused to Pex19p variants indicated on the left. Transformants were assayed for ß-galactosidase expression (A, left panels) and growth on synthetic complete medium lacking His in the presence of 10 mM 3-aminotriazole (3AT) (A, right panels; B). BD-fused peroxins without Pex19p and Pex19p-AD with mock vector showed no self-activation.





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