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Figure 2


Fig. 2. Turnover of Tie2 in HUVECs. [35S]methionine and [35S]cysteine labelled HUVECs were washed and incubated at 37°C for the indicated times in either HUVEC medium alone, or containing pre-clustered Ang1 (800 ng/ml), or Ang2 (800 ng/ml). The cells were lysed, Tie2 immunoprecipitated with an anti-Tie2 antibody, and immunoprecipitates resolved by SDS-PAGE. (A) Representative autoradiograph. (B) Semi-logarithmic plot of the relative Tie2 intensities versus time in unstimulated cells (bullet), in cells stimulated with Ang1 ({diamond}) or Ang2 ({blacksquare}). Each point on the graph is an average value from three to nine independent experiments.





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