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Figure 1


Fig. 1. RT-PCR analysis at 0, 24, 48 and 72 hours of NM2A and NM2B expression levels in knockdown experiments. Data were obtained for untreated, sense, scrambled and antisense cells. (A) Example PCR results are shown for NM2A oligonucleotides and the corresponding ß-actin controls, and NM2B oligonucleotides. The data in each row were taken from the same experiment, and the same gel, but the results have been re-organised for display. (B) Time course of RNA levels for NM2A and NM2B in response to oligonucleotide treatment. RNA levels are normalised to actin mRNA in each extract and to the level at the zero time point. n=2 for NM2B; n=3 for NM2A and the error bars (s.e.m.) are shown for NM2A.





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